Table of Contents
- 1 What precautions should be taken while using pipette?
- 2 How do you calibrate a pipette and burette?
- 3 What are some common pipette handling errors?
- 4 Why do we need to calibrate a burette?
- 5 Why do we need to calibrate pipette?
- 6 How do you calibrate a colorimeter?
- 7 What precautions should be taken when using a pipette?
- 8 Which finger should be dry when using a pipette?
What precautions should be taken while using pipette?
Take Care of Your Pipette
- Have Your Pipette Serviced Every 6–12 Months.
- Check Your Pipette for Damage Daily.
- Clean Your Pipette Each Day Before Use.
- Store Your Pipette Vertically, Using a Pipette Holder.
- Never Put Your Pipette on Its Side With Liquid in the Tip.
- Use Well-Fitting Tips.
How do you calibrate a pipette and burette?
Calibration of a burette and pipette is carried out by measuring the mass of a known volume of water delivered by the burette or the pipette. By knowing the temperature and the density of water at that temperature, the true volume delivered by the burette or the pipette is calculated.
What are the steps for the calibration of burette?
Touch the tip of the buret to the side of a beaker to remove the drop hanging from the tip. After about a minute, to allow for drainage, make an initial reading of the meniscus, estimating the volume to the nearest 0.01 mL. Record the initial reading. Allow the buret to stand for 5 minutes and recheck the reading.
Why do you calibrate a burette?
For careful analytical work, each buret should be calibrated to ensure that the buret markings conform to these standards. Similarly, the tolerance of a 2-mL, class A, transfer pipet is ± 0.006 mL (or 3 ppth). The 2-mL pipet should also be calibrated. If the problem persists, the buret should be replaced.
What are some common pipette handling errors?
Common pipetting errors include:
- Failure to Pre-Wet Pipette Tip.
- Disregarding Temperature.
- Tip Wiping.
- Choosing the Wrong Pipetting Mode.
- Working Too Quickly.
- Pipetting at an Angle.
- Using the Wrong Pipette Tips.
Why do we need to calibrate a burette?
Why is calibrating a pipette important?
Pipette calibration is necessary to prevent inaccuracies in order to attain better results, performance, and longevity of the pipette itself.
What is the aim of calibration of burette?
To calibrate glassware such as burette, pipette, volumetric flask, beaker, and measuring cylinder and list possible errors that could occur in an experiment.
Why do we need to calibrate pipette?
The Importance of a Well-Calibrated Pipette A pipette is used to precisely measure and transfer small volumes of liquid in the laboratory in experiments that demand high accuracy. It is, therefore, necessary to check pipette calibration every few months to ensure accuracy by dispensing the right volumes.
How do you calibrate a colorimeter?
Press and hold the CAL button on the Colorimeter. When the red LED begins to flash, release the CAL button. When the red LED stops flashing, the Colorimeter is calibrated.
What is the purpose of calibrating a volumetric pipette?
Any discrepancy in volumes dispensed may affect the outcomes and reproducibility of an experiment such as qPCR results. It is, therefore, necessary to check pipette calibration every few months to ensure accuracy by dispensing the right volumes.
What is the procedure for calibration of pipettes?
To lay down the procedure for calibration of pipettes, burettes and volumetric flasks used in the Quality control. Calibration of following types of graduated pipettes is performed. Demineralised water is drawn in the pipette and the level of water is accurately adjusted at 0.0 mark.
What precautions should be taken when using a pipette?
* Do not immerse the pipette into the liquid already in the receiving vessel, unless the pipette is a “blow-out” in which you can aspirate and blow out the liquid at the tip of the pipette to assure proper volume has been delivered. Being aware of these precautions would avoid laboratory accidents and inaccurate measurement of solutions.
Which finger should be dry when using a pipette?
The index finger must be dry. Do not use the thumb as it would be more difficult to manipulate. * Do not forget to wipe the sides of the pipette with a tissue or filter paper. This would remove excess liquid clinging to the external sides of the pipette, which may inaccurately add to the total volume of the solution.
Can I Use my Thumb to clean the side of pipette?
Do not use the thumb as it would be more difficult to manipulate. * Do not forget to wipe the sides of the pipette with a tissue or filter paper. This would remove excess liquid clinging to the external sides of the pipette, which may inaccurately add to the total volume of the solution.