Table of Contents
- 1 Why you should not hasten the dispensing of the solution by blowing throughout the mouthpiece?
- 2 Which pipette should be blown out?
- 3 What should not be blown out?
- 4 Why do not use the pipette outside the designated range?
- 5 Why pipette should never be held from its bulb?
- 6 How does a pipette work?
- 7 Is Mohr pipette TC?
- 8 Why is the last drop inside a pipette not blown out?
- 9 Why did mouth pipetting fall out of favor?
- 10 Do you have to blow out a calibrated pipette?
Why you should not hasten the dispensing of the solution by blowing throughout the mouthpiece?
The pipettes are usually designed to be calibrated without blowing out, and with a liquid of similar viscosity as water. Eg water. Blowing them out mucks up the benefit of the calibration.
Which pipette should be blown out?
The serological pipette must be blown out to deliver the entire volume of the pipette and has an etched ring (or pair of rings) near the bulb end of the pipette signifying that it is a blow-out pipette. Serological pipettes have a larger orifice than do the Mohr pipettes and thus drain faster.
Why is the remaining fluid at the delivery tip of a Mohr pipette is not blown out?
The graduations near the tip will not be linear. A pipet that is graduated all the way to the tip is designed so that the liquid contained after delivering is calibrated into the graduations. Thus, never attempt to blow the remaining liquid from a pipet.
What should not be blown out?
A volumetric pipet should not be “blown out” to eject all liquid at the tip because volumetric pipets are calibrated in a manner that takes into account the solution which remains at the tip due to surface tension. It can be placed firmly on the mouth of the pipet.
Why do not use the pipette outside the designated range?
Never use a pipetter outside of its prescribed volume range, it will not pipette accurately and you can compromise the calibration.
Why should not a pipette be held from its bulb?
We should not hold the pipette by the bulb or below the bulb. This is because it is the weakest part of the pipette and holding will put stress through the bulb. If the pipette breaks while being inserted into the filler, sharp pieces of glass usually get embedded in one or both hands of a student.
Why pipette should never be held from its bulb?
Pipette should never be held from its bulb, why? Ans. The heat of our body may expand the glass bulb and introduce an error in the measurement of the volume.
How does a pipette work?
In the air cushion principle, an air cushion separates the liquid in the tip from the pis- ton inside the pipette. The piston moves the air cushion and the liquid is thus taken up into the pipette tip or dispensed out of it. The air cushion thus works like an elas- tic spring, to which the liquid sticks.
What is a Mohr pipette used for?
A Mohr pipette, also known as a graduated pipette, is a type of pipette used to measure the volume of the liquid dispensed, although not as accurately as a volumetric pipette. These use a series of marked lines (as on a graduated cylinder) to indicate the different volumes.
Is Mohr pipette TC?
A Mohr pipette is designed for use as a drain-out pipette. It has a straight tube and graduation marks indicating 0.10 millilitres (0.0035 imp fl oz; 0.0034 US fl oz) changes volume. The designation of whether the pipette is “to deliver” (TD) or “to contain” (TC) is marked on most serological pipettes.
Why is the last drop inside a pipette not blown out?
, Farmer. The last drop inside a pipette is not blown out because a small drop of liquid stays in the pipette on account of surface tension. Secondly, pipettes are made in such a way that the experimental value does not get affected by this drop. If it is blown off, you will get a wrong value.
What is a serological or blow-out pipette?
Serological or ‘blow-out’ pipettes are calibrated so that the last drop of liquid needs to be blown-out of the tip to deliver the full volume of the pipette. (If you are not trying to deliver the full volume of the pipette, you may not have to blow these out.) In our lab, all of the measuring pipettes will be of the blow-out variety.
Why did mouth pipetting fall out of favor?
However, continued accidents and infections in laboratories illustrate, even today, that there is a lack of acceptance of the simple precautionary measured needed (2). By the 1970s, mouth pipetting had fallen out of favor as swanky, mechanically adjustable and cheap pipettes flooded the market (3).
Do you have to blow out a calibrated pipette?
If you are using a glass pipette Calibrated “to deliver” the pipet delivers the correct volume without blowing it out. “To contain” pipettes exist for special purposes and need to be blown or rinsed out to deliver the calibrated volume. Automatic Pipettes usually need to be blown out by pressing the plunger all the way.