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Where does the Ti plasmid come from?
The most common cause is the Ti plasmid (tumor-inducing plasmid), which is carried by soil bacteria of the Agrobacterium group. The most important aspect of the infection is that a specific segment of the Ti plasmid DNA is transferred from the bacteria to the plant.
In which of the following bacterial species has Ti plasmid?
The Ti plasmids belong to the repABC family of replicons, whose members are widely distributed among many species of Alphaproteobacteria (1). This replicon family is composed mainly of extrachromosomal plasmids and some secondary chromosomes.
What is Ti plasmid name the organism where it is found?
An extra-chromosomal DNA which delivers gene of interest into a variety of plants and acts as cloning vector in the host organism is called Ti plasmid. It is present in pathogenic bacterium Agrobacterium tumefaciens.
What is the source of T DNA?
The transfer DNA (abbreviated T-DNA) is the transferred DNA of the tumor-inducing (Ti) plasmid of some species of bacteria such as Agrobacterium tumefaciens and Agrobacterium rhizogenes(actually an Ri plasmid). The T-DNA is transferred from bacterium into the host plant’s nuclear DNA genome.
What is disarmed Ti plasmid?
Abstract. Agrobacterium-mediated plant transformation has been used widely, but there are plants that are recalcitrant to this type of transformation. This transformation method uses bacterial strains harboring a modified tumor-inducing (Ti) plasmid that lacks the transfer DNA (T-DNA) region (disarmed Ti plasmid).
What is opine in Ti plasmid?
Opines are low molecular weight compounds found in plant crown gall tumors or hairy root tumors produced by pathogenic bacteria of the genus Agrobacterium and Rhizobium. Each strain of Agrobacterium and Rhizobium induces and catabolizes a specific set of opines, this set typifying the Ti plasmid and Ri plasmid.
The Ti plasmid is a large conjugative plasmid or megaplasmid of about 200 kb. Virulence genes are responsible for the transfer of T DNA into the host cell and integration of T DNA with host genome. Octopine Ti plasmids produce an opine called octopine (C9H18N4O4).
What is planta method?
Vacuum infiltration, injection of Agrobacterium culture to plant tissues, pollen-tube pathway, floral dip and floral spray are the main methods of in planta transformation. Each of these methods has its own advantages and disadvantages.
How is Ti plasmid used in biotechnology process?
The Ti plasmid vector. In plant genetic engineering, the Ti plasmid can be used to carry foreign genes into plant cells. The Ti plasmid is the disease-causing agent of the soil-borne bacteria Agrobacterium tumefaciens. When the bacteria infect a plant, (more…)
What Planta means?
1 : the back side of the shank of a bird’s leg. 2a : the flattened end of the proleg of a caterpillar. b : a sclerite on the insect pretarsus.