Table of Contents
- 1 What is the UV box used for in gel electrophoresis?
- 2 Why is UV light used to visualize DNA after electrophoresis?
- 3 What is the function of Transilluminator?
- 4 How do you use UV Transilluminator?
- 5 How do you make DNA visible by UV light after gel electrophoresis?
- 6 What is the purpose of ladder in PCR?
- 7 How do you clean UV Transilluminator?
- 8 How does Transilluminator prevent UV light?
What is the UV box used for in gel electrophoresis?
Ultraviolet (UV) transilluminators or UV light boxes are used in biotechnology for visualization of nucleic acids (DNA or RNA) after gel electrophoresis and ethidium bromide staining.
Why is UV light used to visualize DNA after electrophoresis?
Research laboratories commonly use fluorescent DNA stains because they are extremely sensitive, making it easy to quantify small amounts of DNA. In order to visualize the DNA fragments, an ultraviolet (UV) light source (such as a transilluminator) is used to excite the fluorescent molecules.
At which wave length of UV should be used to visualize DNA bands when you utilize UV Transilluminator Why?
We recommend the 312nm wavelength transilluminator for standard gel documentation requirements. 254nm wavelength transilluminators will cause more DNA damage and are suited to other applications such as crosslinking. 254nm bulbs may also be visible through the filter making them unsuitable for gel documentation.
What is the function of Transilluminator?
An ultra-violet (UV) transilluminator is a standard piece of equipment used in life science laboratories for visualization of target DNAs and proteins. The UV transilluminator works by emitting high levels of UV radiation through the viewing surface.
How do you use UV Transilluminator?
The top part of the transilluminator includes the transilluminator glass covered with the solacryl UV-transmissive protective cover.
- Place the lower lid onto the top.
- Lay the upper lid onto the lower lid.
- Place the transilluminator glass into the slot on the upper lid.
Can you see DNA under UV light?
To actually see the DNA products, you MUST use some sort of stain or dye. DNA is colorless and is NOT visible under normal light (or UV light) without a dye or stain.
How do you make DNA visible by UV light after gel electrophoresis?
To make the DNA visible in the gel, ethidium bromide is added to the gel solution and the buffer (it can also be left out of the gel and buffer; staining of the gel can be done in that case after the gel run..).
What is the purpose of ladder in PCR?
When run alongside an unknown PCR product in an agarose gel, the ladder allows you to estimate the size of the unknown fragment by comparing it to the closest band in the ladder lane, like so: Ladder is also run alongside RFLP products to help estimate the size of the restriction fragments.
What is a UV crosslinker?
The Crosslinkers are designed to measure and control the ultraviolet (UV) radiation within the exposure chamber. This same UV sensor feedback measurement system allows you to set UV sample exposure, which automatically deactivates the UV sources when the set UV energy dose has been achieved.
How do you clean UV Transilluminator?
Cleaning and Care of the Transilluminator Clean unit surface with a damp soft cloth or sponge. Never use abrasive cleaners (can damage the UV filter surface). To protect the filter glass and minimize moisture and liquids on the glass, it is recommended that a UV transmitting Gel-Tray is utilized.
How does Transilluminator prevent UV light?
Ultraviolet laboratory rules for use of transilluminators Face and eyes must be covered by wearing an appropriate UV absorbing full face shield. Heavy duty rubber gloves should be worn on the hands. Standard laboratory gloves are not suitable for hand protection from UV.