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What is osmolarity in biology in simple terms?
Definition. Osmolarity is the total solute concentration within a specific volume of a solvent expressed in osmoles per liter (Osm/L) or milliosmoles per liter (mOsm/L). Osmoles are measured concentrations of dissociated ions in a solution that contribute to osmotic pressure.
What is osmolarity of a cell?
Osmolarity is defined as the number of ionic species in molarity that has a characteristic range depending on the species examined. For example, cell culture media are usually adjusted to 290–320 mOsmol to prevent any osmotic stress to cells.
What is urine osmolality test?
An osmolality urine test is performed to measure the concentration of particles in urine. Greater than normal results may indicate conditions such as Addison disease, congestive heart failure or shock.
What is tonicity in biology?
Tonicity is defined as the ability of a solution surrounding a cell to cause that cell to gain or lose water (Urry et al., 2017). While osmolarity is an absolute quantity, tonicity is relative. If two solutions have the same concentration of nonpenetrating solutes, they are considered isotonic.
What is isotonic in biology?
Isotonic solution: A solution that has the same salt concentration as cells and blood.
How do you test for osmolarity?
During a blood test (serum osmolality or plasma osmolality): A health care professional will take a blood sample from a vein in your arm, using a small needle. After the needle is inserted, a small amount of blood will be collected into a test tube or vial. You may feel a little sting when the needle goes in or out.
What does high urine osmolarity mean?
An osmolality test measures the concentration of particles in a solution. In this case, the solution is urine. An osmolality urine test is performed to measure the concentration of particles in urine. Greater than normal results may indicate conditions such as Addison disease, congestive heart failure or shock.
What is transported in exocytosis?
Exocytosis (/ˌɛksoʊsaɪˈtoʊsɪs/) is a form of active transport and bulk transport in which a cell transports molecules (e.g., neurotransmitters and proteins) out of the cell (exo- + cytosis). As an active transport mechanism, exocytosis requires the use of energy to transport material.
How does osmolarity relate to tonicity?
Tonicity is equal to the osmolality less the concentration of these ineffective solutes and provides the correct value to use. Osmolality is a property of a particular solution and is independent of any membrane. Tonicity is a property of a solution in reference to a particular membrane.
How to calculate osmolarity?
A simple equation for calculating the osmolarity of a solution is derived from the molarity of the solution such that: Osmolarity = Molarity × n n is the number of particles or ions present in the solution. For instance a solution of NaCl will dissociate into two ions that are Na+ and Cl-.
What causes high osmolality?
Osmolality increases when you are dehydrated and decreases when you have a fluid buildup. Your body has a unique way to control osmolality. When osmolality increases, it triggers your body to make antidiuretic hormone (ADH). Your kidneys then keep more water inside your body and your urine becomes more concentrated.
What is the normal osmolality?
The normal 24-hour urine osmolality is, on average, 500-800 mOsm/kg of water. Random urine osmolality should average 300-900 mOsm/kg of water. After 12-14 hours of fluid intake restriction, the urine osmolality should exceed 850 mOsm/kg of water.
How do you calculate osmolarity of a solution?
To find the osmolarity of a 0.3\% NaCl solution, you first calculate the molarity of the salt solution and then convert the molarity to osmolarity. Convert percent to molarity: 0.03 \% = 3 grams / 100 ml = 3 grams / 0.1 L = 30 g/L.