Table of Contents
What is a pipette filler?
DESCRIPTION. Use this pipette filler to safely fill glass and plastic pipettes. The three-valve design allows you to release air, draw liquid into the pipette, and accurately regulate or release liquid. Works with all larger sizes of Mohrs, serological, or volumetric pipettes.
What are the advantages of micropipettes how are they used?
Measuring pipettes are calibrated with small divisions and are often adjustable, allowing users to accurately draw up the necessary amount of liquid. Micropipettes are accurate and precise and can transfer measured volumes of microliters of volume.
What is a pipette filler used for in titration?
Titrations are a very accurate experimental technique used to find volumes of acid and alkali solutions that react together. A safety pipette filler should be used to draw up the solution until the bottom of the meniscus is on the line and the solution is then released into a conical flask. …
When inserting the pipette filler or bulb to the pipette one must hold the pipette at?
Regardless of the type of pipette filler, hold the pipette at the top and carefully insert it into the filler by pushing gently. 1. Hold the pipette at the top and carefully insert it into the rubber bulb filler.
Why do we use the pipette filler and not our mouths to fill the pipette?
Do not ever use your mouth to pull the liquid into a pipet. This is the most common method of becoming poisoned in a chemical laboratory or becoming infected in a clinical laboratory. Also, having the bulb attached to the pipet increases the risk of drawing the solution into the bulb. …
What’s the difference between pipettes and micropipettes?
Pipettes and micropipettes are invaluable pieces of laboratory equipment used to draw up, measure, and deliver accurate volumes of liquid. The difference between the two is that micropipettes measure between 1 and 1000µl, while pipettes generally start at 1 milliliter.
Why are micropipettes accurate?
Manufacturers determine the accuracy and precision of micropipettes by using them to transfer defined volumes of distilled water to a drop that is then weighed on an analytical balance. Accuracy is computed from the difference between the actual volume dispensed by the micropipette and the intended volume.
What are the common errors done by technicians while handling pipette?
Common pipetting errors include:
- Failure to Pre-Wet Pipette Tip.
- Disregarding Temperature.
- Tip Wiping.
- Choosing the Wrong Pipetting Mode.
- Working Too Quickly.
- Pipetting at an Angle.
- Using the Wrong Pipette Tips.
Why must a pipette filler always be used when working with a pipette?
The 3-valve pipette filler allows the operator to draw solution up into the pipette, lower the solution level to the calibration mark, and finally drain the solution from the pipette.
How are pipettes important in molecular biology?
Pipettes are an essential laboratory tool used to dispense measured volumes of liquids. Pipettes enable sterile and accurate liquid handling and are commonly used within molecular biology, analytical chemistry and medical tests.